Evaluation of a rapid ELISA technique for detection of circulating antigens of Toxoplasma gondii
نویسندگان
چکیده
منابع مشابه
Detection of Toxoplasma gondii antigens by a dot-immunobinding technique.
A sensitive assay for the detection of antigens of Toxoplasma gondii by spotting samples directly onto nitrocellulose paper was developed. The sensitivity ranged from 10 to 40 pg of antigen diluted in phosphate-buffered saline and 40 to 130 pg of antigen diluted in normal mouse serum, normal human serum, or human cerebrospinal fluid. T. gondii antigen in serum samples taken from mice infected w...
متن کاملevaluation of recombinant sag1 protein for detection of toxoplasma gondii specific immunoglobulin m by elisa test
b ackground: toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. since the aim of this study was to assess the diagnostic utility of recombinant sag1 (rec- sag1) for the detection of toxoplasma -specific igm antibodies in human sera, by an enzyme-linked immunosor...
متن کاملdetection of toxoplasma gondii antigens in sera and urine of experimentally infected mice by capture elisa
background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diagnosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrifi...
متن کاملEvaluation of Recombinant SAG1 Protein for Detection of Toxoplasma gondii Specific Immunoglobulin M by ELISA Test
BACKGROUND Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec-SAG1) for the detection of Toxoplasma-specific IgM antibodies in human sera, by an enzyme-linked immunosorbent...
متن کاملDetection of circulating antigens in sera of rabbits infected with Toxoplasma gondii.
Experiments were performed to investigate the usefulness of an enzyme-linked immunosorbent assay utilizing avidin-biotin interaction as a diagnostic tool for detection of Toxoplasma antigen in blood. The lower limit of sensitivity of the assay by this method was ca. 4 ng/ml, and standard assays provided a linear plot of antigen concentration over a range up to 250 ng/ml. In rabbits inoculated s...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 2008
ISSN: 0385-5600,1348-0421
DOI: 10.1111/j.1348-0421.2008.00020.x